We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Molecular Characterization, Antimicrobial Susceptibility and Salt Tolerance of Aeromonas hydrophila from Fresh, Brackish and Marine fishes.
- Authors
Dahdouh, Basma; Basha, Ola; Khalil, Samy; Tanekhy, Mahmoud
- Abstract
A total number of 170 fishes (100 fresh water, 40 brackish water, and 30 marine water fishes) from different farms in Alexandria, Kafr Elsheikh, and El-Behera governorates were collected from different water salinities. The prevalence of Aeromonas hydrophila was 47% (38% in fresh water fish, 65% brackish water fish, 53.3% in marine water fish). A.hydrophila isolates were tested for pathogenicity by inoculation into blood agar and skimmed milk agar 1% for hemolytic and proteolytic activity. All isolates showed β hemolysis and proteolytic activity. A.hydrophila isolates were inoculated into trypticase soya broth with different NaCl concentration (0.5, 1, 2, 3, 4, 5, and 6%) and turbidity was measured by photometer at wave length 610 nm. The higher NaCl concentration, the lower turbidity was found. Moreover, reduction % of total bacterial count of A.hydrophila in relat ion to different concentration of NaCl in tryptic soya broth (TSB) after 10 fold serial dilutions by surface plating technique was calculated. Hemolysin and aerolysin genes were detected by PCR from the isolates obtained from different sources. A.hydrophila isolates from fresh, brackish and marine fish showed high resistance to Ampicillin (M10), Erythromycin (E15) Nalidixic acid (NA30), and Spectinomycin (SPT10). In contrast, they showed high sensitivity to Enrofloxacin (EF10), Ofloxacin (OFX5) and Gentamicin (CN10). Polymixin (PB300 u) showed high activity against A.hydrophila isolated from brackish and marine fishes only. Doxycycline (DO30) and Nitrofurantoin (F300u) were highly effective against brackish water isolates than others for the field application. Isolation of A.hydrophila from different water salinit ies raises the public health concern and the importance to find suitable methods to control the infection.
- Subjects
AEROMONAS hydrophila; MARINE fish physiology; DIAGNOSTIC use of polymerase chain reaction
- Publication
Alexandria Journal of Veterinary Sciences, 2016, Vol 48, Issue 2, p46
- ISSN
1110-2047
- Publication type
Article
- DOI
10.5455/ajvs.208107