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- Title
当归转录因子 AsMYB44 的克隆与功能研究.
- Authors
刘光瑞; 宗渊; 李云; 曹东; 刘宝龙; 包雪梅; 李建民
- Abstract
In order to study the mechanism of biosynthetic regulation of Angelica sinensis phenolic acids, a MYB transcription factor AsMYB44 gene was cloned from Angelica sinensis and the relevant bioinformatics analysis of the sequence was carried out using biological software. The Agrobacterium tumefaciens-mediated genetic transformation system was overexpressed in Samsun tobacco, and a multi-omics combined analysis of the transgenic tobacco was performed. The CDS (coding sequence) of the AsMYB44 was 903 bp, encoding 300 amino acids, and with the complete SANT and MYB domains. Phylogenetic analysis showed that AsMYB44 had higher homology with Arabidopsis thaliana AtMYB11 (AF062863.1), AtMYB12 (AF062864.1) and Salvia miltiorrhiza SmMYB97 (KF059561.1). The results of transcriptome sequencing showed that the differentially expressed genes were mostly enriched in the phenylpropane synthesis and metabolism pathway in AsMYB44 transgenic tobacco. Expression levels of phenylalanine ammonia lyase gene (PAL), cinnamic acid-4-hydroxyl (C4H), 4-coumaric acid: coenzyme A ligase gene (4CL), caffeic acid 3-O-methyltransferase gene (COMT), cinnamyl alcohol dehydrogenase gene (CAD) and other structural genes were upregulated. Metabolomic analysis showed that the contents of metabolites such as phenolic acids, lipids, alkaloids, flavonoids and other compounds in transgenic tobacco changed significantly. These results indicated that the AsMYB44 transcription factor could positively regulate the metabolic synthesis of phenolic acids, which provided a theoretical basis for the biosynthesis mechanism of phenolic acid secondary metabolites in Angelica sinensis.
- Subjects
PHENYLALANINE ammonia lyase; DONG quai; GENE expression; PHENOLIC acids; MOLECULAR cloning; HYDROXYCINNAMIC acids
- Publication
Acta Agriculturae Zhejiangensis, 2023, Vol 35, Issue 6, p1253
- ISSN
1004-1524
- Publication type
Article
- DOI
10.3969/j.issn.1004-1524.2023.06.03