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- Title
半滑舌鳎体表溃疡症细菌性病原的分离鉴定 及组织病理学研究.
- Authors
吴雅婷; 汪笑宇; 汤青平; 武尊; 高浩峰; 何学欣; 杨张磊; 贾旭颖; 邵蓬
- Abstract
To investigate the causes of ulcers on the surface and a large number of deaths of the Cynoglossus semilaevis in a certain farm, bacterial isolation was performed from the liver, spleen and kidney of the diseased fish, and dominant strains 8-0634 and 8-0635 were obtained from the spleen and kidney, respectively. The strains were used for artificial infection experiments, and the results showed that only strain 8-0635 could cause the death of the Cynoglossus semilaevis and the symptoms were scale off, ascites, intestinal congestion to the outward, spleen, kidney and other organs atrophy congestion. A large amount of inflammatory cell infiltration occured between tissues. According to the results of Gram staining and physiological and biochemical experiments, the strain was initially identified as Vibrio harveyi, and then the phylogenetic tree was constructed based on the housekeeping gene and 16S rRNA sequence to further identify the pathogenic bacteria as Vibrio harveyi. The pathological description and drug susceptibility test were performed. The results of drug susceptibility test showed that the pathogen was high sensitivity to various drugs such as cefoperazone, florfenicol, chloramphenicol and ampicillin. This study provided a theoretical basis for disease prevention and control of fish infected with Vibrio harveyi.
- Subjects
GRAM'S stain; CYNOGLOSSUS; PATHOGENIC bacteria; SPLEEN; PREVENTIVE medicine; VIBRIO harveyi; ORGANS (Anatomy); KIDNEYS
- Publication
Journal of Agricultural Science & Technology (1008-0864), 2023, Vol 25, Issue 6, p135
- ISSN
1008-0864
- Publication type
Article
- DOI
10.13304/j.nykjdb.2021.1108