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- Title
DNA Fingerprinting of Acer truncatum Clones Using SRAP Markers.
- Authors
Xinling QI; Zhen FENG; Qian QIAO
- Abstract
[Objective] This study aimed to establish DNA fingerprints of 23 Acer truncatum clones, thus providing the theoretical basis for selection, classification and identification of A. truncatum varieties. [Method] Sixteen pairs of SRAP primers with rich polymorphism and high specificity were used to establish DNA fingerprints. [Result] A total of 223 b a d s were amplified with 16 primer pairs, including 197 polymorphic bands. Averagely 13.9 loci and 12.3 polymorphic loci were amplified with each primer pair. The average percentage of polymorphic loci reached 88. 34%. [ Conclusion] The classification result drawn by cluster analysis is consistent with that obtained based on main characteristics a d genetic relationships of A. truncatum clones. By using DNA fingerprints established with primer pairs ME1-EM4 and ME2-EM1, 23 A. truncatum clones can be effectively distinguished, and the confidence probability is greater than 99.99%.
- Subjects
DNA fingerprinting; LOCUS (Genetics); GENETIC polymorphisms; DNA primers; CLUSTER analysis (Statistics)
- Publication
Agricultural Biotechnology (2164-4993), 2016, Vol 5, Issue 4, p26
- ISSN
2164-4993
- Publication type
Article