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- Title
The C-terminal regions of YidC from Rhodopirellula baltica and Oceanicaulis alexandrii bind to ribosomes and partially substitute for SRP receptor function in Escherichia coli.
- Authors
Seitl, Ines; Wickles, Stephan; Beckmann, Roland; Kuhn, Andreas; Kiefer, Dorothee
- Abstract
The marine Gram-negative bacteria Rhodopirellula baltica and Oceanicaulis alexandrii have, in contrast to Escherichia coli, membrane insertases with extended positively charged C-terminal regions similar to the YidC homologues in mitochondria and Gram-positive bacteria. We have found that chimeric forms of E. coli YidC fused to the C-terminal YidC regions from the marine bacteria mediate binding of YidC to ribosomes and therefore may have a functional role for targeting a nascent protein to the membrane. Here, we show in E. coli that an extended C-terminal region of YidC can compensate for a loss of SRP-receptor function in vivo. Furthermore, the enhanced affinity of the ribosome to the chimeric YidC allows the isolation of a ribosome nascent chain complex together with the C-terminally elongated YidC chimera. This complex was visualized at 8.6 Å by cryo-electron microscopy and shows a close contact of the ribosome and a YidC monomer.
- Subjects
PROTEOBACTERIA; C-terminal binding proteins; MEMBRANE proteins; BACTERIAL proteins; MITOCHONDRIA; GRAM-negative bacteria; ESCHERICHIA coli
- Publication
Molecular Microbiology, 2014, Vol 91, Issue 2, p408
- ISSN
0950-382X
- Publication type
Article
- DOI
10.1111/mmi.12465