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- Title
Alternative mutations of BRAF, RET and NTRK1 are associated with similar but distinct gene expression patterns in papillary thyroid cancer.
- Authors
Frattini, Milo; Ferrario, Cristina; Bressan, Paola; Balestra, Debora; de Cecco, Loris; Mondellini, Piera; Bongarzone, Italia; Collini, Paola; Gariboldi, Manuela; Pilotti, Silvana; Pierotti, Marco A.; Greco, Angela
- Abstract
Papillary thyroid carcinoma (PTC) is associated with RET and NTRK1 rearrangements and BRAF mutations. A series of 60 PTCs collected in a single center from Italian patients were histologically re-examined and subclassified as well differentiated or tall cell variant. The sample collection was analysed for the presence of all the reported PTC-associated genetic alterations through DNA or cDNA amplification, followed by automated sequencing. The analysis of exons 11 and 15 of BRAF gene revealed the T1796A (V599E) mutation in 32%of cases, and this alteration is significantly associated with PTC tall cell variant. Oncogenic rearrangements of RET and NTRK1 receptors were found in 33 and 5%of cases, respectively. No Ras mutations were detected. Overall, genetic alterations were detected in two-thirds of samples, and in no single case more than one mutational event was found simultaneously. Gene expression profiling of a subset of 31 tumors performed using cDNA microarray chips showed no strong differences in global gene expression among the different cases. However, a supervised analysis of the obtained data identified a subset of genes differentially expressed in tumors carrying BRAF mutation or RTK rearrangement.Oncogene (2004) 23, 7436-7440. doi:10.1038/sj.onc.1207980 Published online 26 July 2004
- Subjects
ITALY; THYROID cancer; GENETIC mutation; GENES; CANCER patients; ONCOLOGY
- Publication
Oncogene, 2004, Vol 23, Issue 44, p7436
- ISSN
0950-9232
- Publication type
Article
- DOI
10.1038/sj.onc.1207980