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- Title
In Vitro Biofilm Formation on Zirconia Implant Surfaces Treated with Femtosecond and Nanosecond Lasers.
- Authors
Bihn, Soo Kyum; Son, Keunbada; Son, Young-Tak; Dahal, Ram Hari; Kim, Shukho; Kim, Jungmin; Hwang, Jun Ho; Kwon, Sung-Min; Lee, Jong Hoon; Kim, Hyun Deok; Lee, Jae-Mok; Jin, Myoung-Uk; Lee, Kyu-Bok
- Abstract
(1) Background: The purpose of this study was to evaluate how a zirconia implant surface treated with laser technology affects the degree of biofilm formation. (2) Methods: Experimental titanium (Ti) disks were produced that were sandblasted with large grit and acid-etched (T), and they were compared with zirconia (ZrO2) discs with a machined (M) surface topography; a hydrophilic surface topography with a femtosecond laser (HF); and a hydrophobic surface topography with a nanosecond laser (HN) (N = 12 per surface group). An in vitro three-species biofilm sample (Aggregatibacter actinomycetemcomitans (Aa), Porphyromonas gingivalis (Pg), Prevotella intermedia (Pi)) was applied to each disc type, and bacterial adhesion was assessed after 48 and 72 h of incubation using an anaerobic flow chamber model. Statistical significance was determined using the Kruskal–Wallis H test, with Bonferroni correction used for the post-hoc test (α = 0.05). (3) Results: Compared to the T group, the M group exhibited more than twice as many viable bacterial counts in the three-species biofilm samples (p < 0.05). In comparison to the T group, the HF group had significantly higher viable bacterial counts in certain biofilm samples at 48 h (Aa and Pi) and 72 h (Pi) (p < 0.05). The HN group had higher viable bacterial counts in Pi at 48 h (5400 CFU/mL, p < 0.05) than the T group (4500 CFU/mL), while showing significantly lower viable bacterial counts in Pg at both 48 (3010 CFU/mL) and 72 h (3190 CFU/mL) (p < 0.05). (4) Conclusions: The surface treatment method for zirconia discs greatly influences biofilm formation. Notably, hydrophobic surface treatment using a nanosecond laser was particularly effective at inhibiting Pg growth.
- Subjects
FEMTOSECOND lasers; ZIRCONIUM oxide; BIOFILMS; HYDROPHOBIC surfaces; HYDROPHILIC surfaces; SURFACE topography
- Publication
Journal of Functional Biomaterials, 2023, Vol 14, Issue 10, p486
- ISSN
2079-4983
- Publication type
Article
- DOI
10.3390/jfb14100486