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- Title
Proteomic identification of small, copper-responsive proteins in germinating embryos of Oryza sativa.
- Authors
Hongxiao Zhang; Chunlan Lian; Zhenguo Shen
- Abstract
Background and Aims Although copper (Cu) is an essential micronutrient for plants and algae, excess Cu is toxic to most plants and can cause a wide range of deleterious effects. To investigate the response of rice (Oryza sativa) to Cu stress, a proteomic approach was used to analyse Cu stress-induced changes in the expression of low molecular-weight proteins in germinating rice seed embryos. Methods Rice seeds were germinated in the presence or absence of 200 µm Cu for 6 d, and embryos, including newly formed shoots and radicles, were isolated. After proteins were extracted from the germinating embryos and separated by two-dimensional PAGE, 16 proteins in the 6- to 25-kDa range were identified using MALDI-TOF mass spectrometry. Key Results and Conclusions Thirteen of the proteins identified, including metallothionein-like protein, membrane-associated protein-like protein, putative wall-associated protein kinase, pathogenesis-related proteins and the putative small GTP-binding protein Rab2, were up-regulated by Cu stress. Three proteins, a putative small cytochrome P450 (CYP90D2), a putative thioredoxin and a putative GTPase, were down-regulated by Cu stress. As far as is known, this study provides the first proteomic evidence that metallothionein and CYP90D2 are Cu-responsive proteins in plants. These findings may lead to a better understanding of plant molecular responses to toxic metal exposure.
- Subjects
PLANT embryology; RICE; PLANT nutrients; MICRONUTRIENTS; PROTEOMICS; COPPER poisoning; MATRIX-assisted laser desorption-ionization
- Publication
Annals of Botany, 2009, Vol 103, Issue 6, p923
- ISSN
0305-7364
- Publication type
Article
- DOI
10.1093/aob/mcp012