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- Title
Effects of Ethyl Acetate Extract of Phyllanthus reticulatus Leaves on Autophagy-related Proteins Beclin-1, ATG5 and LC3 in Nude Mice Xenograft Model of HCC.
- Authors
Zhipeng XU; Chenyan LIANG; Cuiliu PAN; Yunli TANG; Jianfang FENG; Tong HE
- Abstract
Objectives: To explore the effects of ethyl acetate extract of Phyllanthus reticulatus leaves on autophagy-related proteins Beclin-1, ATG5 and LC3 by immunohistochemistry, and to preliminarily explore their eefects on autophagy. Methods: BEL-7404 Hepatocellular Carcinoma ( HCC) nude mice model was established, and blank group ( same volume of pure water), positive control group (20 mg/kg fluoroura- cil), high dose drug group (600 mg/kg), and medium dose drug group (300 mg/kg), and low dose drug group (150 mg/kg) were set up. Ater 2 weeks of intragastric administration, the nude mice were sacrificed, and the tumor tissues were taken out, proceesed by immunohisto-chemistry, and then made into paraffin sections. Photos were taken under an optica microscope (10 x40), and evaluation and analysis were performed with the aid of the Image-Pro Plus 6.0 image analysis software. Diiferences were calculated using SPSS 20.0 software. The effects of drugs on autophagy-related proteins LC3, Beclin-1 and ATG5 were observed. Results: Compared with the blak group, the medium and high dose groups of ethyl acetate extract of P. reticulatus leaves had the eefect of promoting the increase of autophagy-related proteins LC3, Beclin-1 and ATG5 ( P < 0. 05 ). However, there was no significant diference between the low dose group of ethyl acetate extract of P. reticulatus leaves and the blank group ( P j 0. 05 ). Conclusions: The ethyl acetate extract of P. reticulatus leaves has a promoting eefect on autophagy-related proteins LC3, Beclin-1, and ATG5.
- Subjects
ANIMAL disease models; LABORATORY mice; PHYLLANTHUS; PROTEINS; ETHYL group; ETHYL acetate
- Publication
Medicinal Plant, 2022, Vol 13, Issue 3, p43
- ISSN
2152-3924
- Publication type
Article
- DOI
10.19600/j/cnki.issn2152-3924.2022.03.011