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- Title
Comparison of conventional and rapid methods for determination of total aerobic mesophilic microorganisms and Enterobacteriaceae in poultry products.
- Authors
ERTUĞRUL, Çetin; ÇAKIR, İbrahim
- Abstract
Objective: In this study, it was aimed to compare the conventional and rapid test methods in determining the numbers of both total aerobic mesophilic microorganizm and Enterobacteriaceae in totally 123 poultry products which were both whole carcass and mechanically separated. Methods: In In the study, it was simultaneously used ISO 4833:2003 conventional method and TEMPO TVC rapid test method for determining the number of total aerobic mesophilic microorganism, as well as ISO 21528-2:2004 conventional method and TEMPO EB rapid test method for the enumeration of Enterobacteriaceae. According to this, it was statistically evaluated the results belonging to 100 samples in a total of aerobic mesophilic microorganism count and also 85 samples in Enterobacteriaceae count. Descriptive statistical test and F-test were performed by using office excel 2007 Software (Microsoft, Redmond, USA) at the statistical comparison of the conventional and rapid test methods. In addition, linear regression and Pearson correlation analyses were performed by using MINITAB 16 software (Minitab Inc., State College, TX, USA). Results: According to the results of the study, it was determined that there was no statistically significant difference between the conventional ISO 4833:2003 and TEMPO TVC methods with respect the accuracy of total aerobic mesophilic bacteria count results. Similarly, it was found that there was also no statistically significant difference between the conventional ISO 21528-2:2004 and TEMPO EB methods in terms of the accuracy of Enterobacteriaceae count results. Conclusion: It was concluded that microbiological analysis performed by TEMPO rapid test system is more advantageous, because it is significantly decreased duration of analysis, analysis cost, ease of operation and risk of contamination according to the conventional ISO methods both the number of total aerobic mesophilic microorganism and Enterobacteriaceae counts.
- Subjects
ENTEROBACTERIACEAE; POULTRY products; F-test (Mathematical statistics)
- Publication
Turkish Bulletin of Hygiene & Experimental Biology / Türk Hijyen ve Deneysel Biyoloji, 2016, Vol 73, Issue 4, p379
- ISSN
0377-9777
- Publication type
Article
- DOI
10.5505/TurkHijyen.2016.98470