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- Title
Indirect ELISA for diagnosing bovine fasciolosis from milk samples.
- Authors
Gómez, Mónica Uruburu; Bedoya Blandon, Juan Carlos; Velásquez Trujillo, Luz Elena
- Abstract
We standardized an ELISA test for the detection of anti-F. hepatica antibodies in bovine milk. A total of 80 samples were used, of which 44 were used as positive controls and 36 as negative controls. Milk samples from bovines presenting paramphistomidae eggs in feces were used as cross-reactivity controls. Sensitivity and specificity of the diagnostic test were 80 and 72,2%, respectively, with 95% confidence interval. These results are comparable with those reported in studies conducted in other countries. We propose this standardized test as an alternative diagnostic and screening tool in epidemiological studies of fasciolosis.
- Subjects
MILK quality; MILK contamination; FASCIOLIASIS; ENZYME-linked immunosorbent assay; PARAMPHISTOMIDAE; DIAGNOSIS
- Publication
CES Medicina Veterinaria & Zootecnica, 2013, Vol 8, Issue 2, p93
- ISSN
1900-9607
- Publication type
Article