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- Title
Albumins and their processing machinery are hijacked for cyclic peptides in sunflower.
- Authors
Mylne, Joshua S.; Colgrave, Michelle L.; Daly, Norelle L.; Chanson, Aurelie H.; Elliott, Alysha G.; McCallum, Emily J.; Jones, Alun; Craik, David J.
- Abstract
The cyclic peptide sunflower trypsin inhibitor 1 (SFTI-1) blocks trypsin and is a promising drug lead and protein engineering scaffold. We show that SFTI-1 and the newfound SFT-L1 are buried within PawS1 and PawS2, precursors for seed storage protein albumins. Proalbumins are matured by asparaginyl endopeptidase, which we show is required to liberate both ends of SFTI-1 as well as to mature PawS1 albumin. Thus, these peptides emerge from within an albumin precursor by the action of albumin's own processing enzyme.
- Subjects
SUNFLOWER seeds; TRYPSIN inhibitors; TRYPSIN; PROTEIN engineering; ALBUMINS; PEPTIDES; ENDOPEPTIDASES
- Publication
Nature Chemical Biology, 2011, Vol 7, Issue 5, p257
- ISSN
1552-4450
- Publication type
Article
- DOI
10.1038/nchembio.542