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- Title
Endometrial Mesenchymal Stem Cells Isolated from Menstrual Blood by Adherence.
- Authors
Du, Xue; Yuan, Qing; Qu, Ye; Zhou, Yuan; Bei, Jia
- Abstract
Objective. To find a convenient and efficient way to isolate MSCs from human menstrual blood and to investigate their biological characteristics, proliferative capacity, and secretion levels. Methods. MSCs were isolated from menstrual blood of 3 healthy women using adherence. Cell immunological phenotype was examined by flow cytometry; the adipogenic, osteogenic, and chondrogenic differentiation of MSCs was examined by Oil-Red-O staining, ALP staining, and Alcian Blue staining, respectively; and the secretion of cytokines, including vascular endothelial growth factor (VEGF), hepatocyte growth factor (HGF), and insulin-like growth factor-1 (IGF-1), was detected using enzyme-linked immunosorbent assay. Results. MB-MSCs were successfully isolated from human menstrual blood using adherence. They were positive for CD73, CD105, CD29, and CD44, but negative for CD31 and CD45. The differentiated MB-MSCs were positive for ALP staining, Oil-Red-O staining, and Alcian Blue staining. In addition, they could secrete antiapoptotic cytokines, such as VEGF, IGF-1, and HGF. Conclusion. It is feasible to isolate MSCs from human menstrual blood, thus avoiding invasive procedures and ethical controversies. Adherence could be a promising alternative to the density gradient centrifugation for the isolation of MSCs from menstrual blood.
- Subjects
MESENCHYMAL stem cells; ENDOMETRIUM physiology; MENSTRUATION; IMMUNE adherence reaction; CELL proliferation; ETHICAL problems; DENSITY gradient centrifugation
- Publication
Stem Cells International, 2015, p1
- ISSN
1687-966X
- Publication type
Article
- DOI
10.1155/2016/3573846