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- Title
Comparación de tres sitios anatómicos de extracción de muestras de medula ósea para el diagnóstico de leishmaniosis canina.
- Authors
Maidana, H. R.; Llano, E. G.; Báez, A. D.; Cabrera, W. R.; López, J. E.
- Abstract
The objectives of this research were to compare three extraction sites of canine bone marrow: sternum, iliac crest and ventral third of the ribs, and to evaluate advantages and anatomical difficulties, volume of the sample, patient's tolerance and cellularity of each region. For this purpose, 10 dogs underwent bone marrow harvesting. Smears were fixed with alcohol, stained with Giemsa, and observed by means of light microscopy with immersion objective to identify cell type for each sampled region. The ventral third of the ribs X, XI and XII may be considered the best site to obtain bone marrow samples, because the superficial position of ribs makes them easy to be located and allows the correct guiding of the needle, minimizing the risks of accidental injury to other organs. Also, the thin cortical bone layer allows better penetration of the needle and a sufficient volume of sample to prepare the smear. The needle puncture is well tolerated and the practice becomes a quick and safe technique for both animal and operator.
- Subjects
BONE marrow; LABORATORY dogs; STERNUM; IMMERSION lithography; RIB cage
- Publication
Revista Veterinaria, 2010, Vol 21, Issue 2, p144
- ISSN
1668-4834
- Publication type
Article
- DOI
10.30972/vet.2121941