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- Title
A Simple, Rapid and Non-Radiolabeled Immune Assay to Detect Anti-AChR Antibodies in Myasthenia Gravis.
- Authors
Bokoliya, Suresh; Patil, Shripad; Nagappa, Madhu; Taly, Arun
- Abstract
Objective To assess the practicality of dot-blot testing for rapid and sensitive detection of the antiacetylcholine receptor (anti-AChR) antibodies in myasthenia gravis (MG). Methods In this case-control study, we tested serum specimens of 85 patients with MG, 85 healthy control individuals, and 85 patients without MG who have other autoimmune and neurological illnesses. All the serum specimens were tested for anti-AChR antibodies using 3 assays: in-house enzyme-linked immunosorbent assay (ELISA), the dot-blot assay, and commercial ELISA. Results In-house ELISA, commercial ELISA, and dot-blot test results were positive for anti-AChR antibodies in 65 (76.5%) patients with MG. The results of all 3 tests were negative for anti-AChR antibodies in healthy controls and patients without MG. We observed perfect concordance (K = 1, P <.001) between all 3 tests. In-house ELISA correlated significantly (r = 0.873, P <.001) with commercial ELISA. In-house ELISA and the dot-blot test demonstrated similar diagnostic performance in detecting anti-AChR antibodies. Conclusions The dot-blot assay is a simple, nonradioactive immune assay for rapid detection of anti-AChR antibodies in MG.
- Subjects
CHOLINERGIC receptors; AUTOANTIBODIES; CONFIDENCE intervals; STATISTICAL correlation; ENZYME-linked immunosorbent assay; IMMUNOASSAY; IMMUNOBLOTTING; MYASTHENIA gravis; RESEARCH funding; CASE-control method; DATA analysis software; DESCRIPTIVE statistics
- Publication
Laboratory Medicine, 2019, Vol 50, Issue 3, p229
- ISSN
0007-5027
- Publication type
Article
- DOI
10.1093/labmed/lmy038