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- Title
Isotopologous Organotellurium Probes Reveal Dynamic Hypoxia In Vivo with Cellular Resolution.
- Authors
Edgar, Landon J.; Vellanki, Ravi N.; McKee, Trevor D.; Hedley, David; Wouters, Bradly G.; Nitz, Mark
- Abstract
Changes in the oxygenation state of microenvironments within solid tumors are associated with the development of aggressive cancer phenotypes. Factors that influence cellular hypoxia have been characterized; however, methods for measuring the dynamics of oxygenation at a cellular level in vivo have been elusive. We report a series of tellurium-containing isotopologous probes for cellular hypoxia compatible with mass cytometry (MC)-technology that allows for highly parametric interrogation of single cells based on atomic mass spectrometry. Sequential labeling with the isotopologous probes (SLIP) in pancreatic tumor xenograft models revealed changes in cellular oxygenation over time which correlated with the distance from vasculature, the proliferation of cell populations, and proximity to necrosis. SLIP allows for capture of spatial and temporal dynamics in vivo using enzyme activated probes.
- Subjects
ORGANOTELLURIUM compounds; OXYGENATION (Chemistry); MASS spectrometry; ENZYME activation; XENOGRAFTS; CHEMICAL reactions
- Publication
Angewandte Chemie International Edition, 2016, Vol 55, Issue 42, p13159
- ISSN
1433-7851
- Publication type
Article
- DOI
10.1002/anie.201607483