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- Title
Black Aspergillus as the main causative agents of otomycosis in Yasuj south west of Iran.
- Authors
Nouripour-sisakht, Sadegh; Gharaghany, Maral; Sabz, Gholamabbas
- Abstract
Introduction: As a common condition throughout the world, otomycosis is an acute, sub-acute or chronic superficial mycotic infection of external auditory canal caused by a wide range of fungal species. In this study we aimed to identify fungal agents isolated from patients clinically suspected to otomycosis. Materials and methods: External ear canal samples were taken from 275 patients who referred to the outpatient department of Shahid- Mofatteh Clinic in Yasuj City, southwest Iran. The collected samples were examined by both direct microscopy and culture. DNA of all isolated fungi were extracted with glass-bead disruption followed by ITS-PCR-RFLP analysis for identification of yeasts and ß-tubulin sequencing for identification of Aspergillus species or other molds. Results: A total of 275 patients suspected to otomycosis were included in this study from which 128 patients were positive on KOH preparation and 144 samples consisted of 61 (42.3%) males and 83 (57.7%) females were positive in culture. Predominant predisposing factor was selfcleaning of external ear with unhygienic tools and housewives were the main occupation. The most common isolated fungi were Aspergillus species (n=120) including 73 isolates of Aspergillus section nigri, 43 of section flavi, 3 of section terrei and one of section fumigati. 34 isolates were Candida species including C. parapsilosis (n=22), C. albicans (n=12) and C. tropicalis (n=1). 8.3% of patients had mix infection of two or three species. Conclusion: As clinical feature of otomycosis are not specific, laboratory diagnosis is important to know the exact etiology of otomycosis for appropriate antifungal therapy. Further research is needed to determine susceptibility of fungal agents to antifungals and targeted treatment.
- Subjects
IRAN; ASPERGILLUS; CONFERENCES &; conventions; EAR; MYCOSES
- Publication
Current Medical Mycology, 2018, Vol 4, p69
- ISSN
2423-3439
- Publication type
Article
- DOI
10.18502/cmm.4.S1.2018.180