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- Title
Serum Amyloid P-Component-Mediated Inhibition of the Uptake of Mycobacterium tuberculosis by Macrophages, In Vitro.
- Authors
Kaur, S.; Singh, P.P.
- Abstract
The effect of purified mouse serum amyloid P-component (SAP) treatment of mouse alveolar macrophages (AMs) on their uptake of Mycobacterium tuberculosis Erdman was investigated, in vitro. SAP (0.5-50.0 µg/ml), in a concentrationdependent manner, inhibited the M. tuberculosis uptake by the AMs; maximum inhibition (33.43%) occurred at 10.0 µg/ml. The inhibition of uptake could be observed as early as 30 min after the incubation of AMs with 10.0 µg/ml SAP; however, an incubation of 60 min induced maximum inhibition beyond which the response became static. The SAP-mediated decreased uptake of M. tuberculosis also resulted in their reduced intramacrophage growth as determined by colonyforming unit counts. SAP inhibited the uptake of mycobacteria in the presence of Ca2+, and at pH = 5.6, the inhibition was abrogated. Deglycosylation of purified SAP with N-glycanase, and not with O-glycanase, blocked the SAPmediated inhibition of the uptake. Heat-inactivated (80 °C; 1 h; pH 7.0) SAP did not inhibit the uptake of M. tuberculosis by AMs. These data, apparently for the first time, indicate that purified mouse SAP, in a divalent cation- and N-linked oligosaccharide glycosylation-dependent manner, inhibited the in vitro uptake of M. tuberculosis Erdman by mouse AMs, which was also associated with their reduced intracellular growth.
- Subjects
MYCOBACTERIUM tuberculosis; MACROPHAGES; TUBERCULOSIS; MYCOBACTERIAL diseases; LUNG diseases; IMMUNOLOGY
- Publication
Scandinavian Journal of Immunology, 2004, Vol 59, Issue 5, p425
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1111/j.0300-9475.2004.01412.x