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- Title
Anti-Tumour Activity of Idiotype-Specific, MHC-Restricted Th1 and Th2 Clones <em>In Vitro</em> and <em>In Vivo</em>.
- Authors
Lauritzsen, G. F.; Weiss, S.; Bogen, B.
- Abstract
Idiotypes (Id) can serve as individual markers on B cells; therefore, cytotoxic Id-specific T cells may play a significant role in immunological surveillance of Id+ B-cell tumours. We have investigated the antitumour activity of CD4+ BALB/c Th1 and Th2 clones which recognize a processed Id of the syngeneic &lembda;2315 L chain in the context of the class II MHC molecule I-Ed. Id-specific T cells and A20/46 B lymphoma cells transfected with the &lembda;2315 gene were injected s.c. into the same site of BALB/c mice (Winn assay). The results show that both Th1 and Th2 clones can protect against tumour development. The protection was Id-specific because T cells did not influence tumour development by an A20/46 B lymphoma cell line transfected with the pSV2neo expression vector alone. In vitro studies showed that the Th1 clones were cytotoxic to &lembda;2315-transfected B lymphoma cells; by contrast, the Th2 clone was not cytotoxic in 51Cr-release assay even though the Th2 cells inhibited the growth of &lembda;2315 B lymphoma cells. The antilymphoma properties of both the Th1 and Th2 clones appear to involve as yet undefined cytotoxic and growth inhibiting molecules.
- Subjects
IDIOTYPIC networks; B cells; TUMORS; LYMPHOMAS; LEUCOCYTES; CELL lines
- Publication
Scandinavian Journal of Immunology, 1993, Vol 37, Issue 1, p77
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1111/j.1365-3083.1993.tb01668.x