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- Title
Adhesion Molecules Involved in the Interaction of LGL/NK Cells and Human Endothelial Cells Stimulated with <em>Salmonella</em> Bacteria.
- Authors
Pinola, M.; Saksela, E.
- Abstract
Previously we showed that pretreatment of LGL/NK or HUVE cells with Salmonella bacteria augments the adhesion of LGL/NK cells to endothelium. Here we analyse the roles of HU VEC adhesion molecules VCAM-I, ICAM-1 and E-selectin, and the counter-receptors VLA-4, LFA-1 and SLex in the increase of LGL/NK adhesion to HUVEC, stimulated with Salmonella Minnesota mR595 bacteria. LPS or TNF-α. On Salmonella-stimulated HUVEC, VCAM-1 and ICAM-1 were the major binding structures involved, and their effect was additive in monoclonal antibody inhibition experiments. We could demonstrate the induction of both structures on cultured HUVEC after 24 h of Salmonella stimulation in flow cytometric analysis. For Salmonella-stimulated LGL/NK, the principal binding structure was LFA-1, Stimulation of LGL/NK cells did not alter the expression of the adhesion structures (subunits CDlla/CDl8, CD49d/CD29), as determined by flow cytometric analysis, and thus the increased adherence is presumably produced by an increased avidity of the receptors on LGL/NK cells. Pretreatment of endothelium or lymphocytes with various stimuli, including Salmonella bacteria or LPS, leads to an activation state which provides for characteristic anchorage sites for the increased migration of LGL/NK cells towards the site of inflammation.
- Subjects
KILLER cells; ADHESION; SALMONELLA; ENDOTHELIUM; BACTERIAL adhesion; MEDICAL research
- Publication
Scandinavian Journal of Immunology, 1992, Vol 36, Issue 5, p671
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1111/j.1365-3083.1992.tb03127.x