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- Title
Triplex real-time polymerase chain reaction assay for detection and quantification of norovirus ( GI and GII) and sapovirus.
- Authors
Niwa, Shoichi; Tsukagoshi, Hiroyuki; Ishioka, Taisei; Sasaki, Yoshiko; Yoshizumi, Masakazu; Morita, Yukio; Kimura, Hirokazu; Kozawa, Kunihisa
- Abstract
ABSTRACT To improve detection of norovirus (NoVGI, NoVGII) and sapovirus (SaV), a simultaneous quantitative RT-PCR method was established. This triplex real-time PCR method was evaluated using a combination of optimized specific primers and probes. The performance of the developed PCR assay was equivalent to that of monoplex real-time PCR across a broad dynamic range of 102-107 copies/assay using plasmid DNA standards. The limit of detection was 102 copies/assay. The quantitative value was comparable with that of monoplex real-time PCR of stool samples. Our triplex real-time PCR is useful for detection of NoV and SaV infections.
- Subjects
NOROVIRUSES; CALICIVIRUSES; DNA primers; PLASMIDS; POLYMERASE chain reaction; COMPARATIVE studies
- Publication
Microbiology & Immunology, 2014, Vol 58, Issue 1, p68
- ISSN
0385-5600
- Publication type
Article
- DOI
10.1111/1348-0421.12107