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- Title
Live non-invasive Shigella dysenteriae 1 strain induces homologous protective immunity in a guinea pig colitis model.
- Authors
Barman, Soumik; Kumar, Ranajit; Chowdhury, Goutam; Rani Saha, Dhira; Wajima, Takeaki; Hamabata, Takashi; Ramamurthy, Thandavarayan; Balakrish Nair, Gopinath; Takeda, Yoshifumi; Koley, Hemanta
- Abstract
ABSTRACT A non-invasive live transconjugant Shigella hybrid (LTSHΔ stx) strain was constructed from a Shiga toxin gene deleted mutant of Shigella dysenteriae 1 by introducing a plasmid vector pPR1347 that carried a lipopolysaccharide biosynthesis gene ( rfb and rfc) of Salmonella typhimurium. In guinea pigs, four successive oral administrations of LTSH Δ stx showed complete protection against rectal challenge with wild type S. dysenteriae 1 strain. Exponential increase of the serum IgG and IgA titer against lipopolysaccharide of LTSH Δ stx was observed during immunization, peaked on day 28 and remained at that level until day 35 after the initiation of the immunization. In intestinal lavage of the immunized animals, significant increase of IgA titer against lipopolysaccharide of LTSH Δ stx was also observed. These data suggested that LTSH Δ stx could be a useful candidate to induce protective immunity against S. dysenteriae 1 infection.
- Subjects
SHIGELLA; COLITIS; IMMUNOREGULATION; GUINEA pigs as laboratory animals; ENDOTOXINS; GENETIC mutation; IMMUNIZATION; BIOSYNTHESIS; SALMONELLA typhimurium
- Publication
Microbiology & Immunology, 2011, Vol 55, Issue 10, p683
- ISSN
0385-5600
- Publication type
Article
- DOI
10.1111/j.1348-0421.2011.00371.x