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- Title
Synergistic effect of tetrandrine and ethidium bromide against methicillin-resistant Staphylococcus aureus (MRSA).
- Authors
Young-Seob Lee; Sin-Hee Han; Su-Hwan Lee; Young-Guk Kim; Chung-Berm Park; Ok-Hwa Kang; Joon-Ho Keum; Sung-Bae Kim; Su-Hyun Mun; Dong-Won Shin; Dong-Yeul Kwon
- Abstract
Methicillin-resistant Staphylococcus aureus (MRSA) along with other resistant bacteria have become a significant social and clinical problem. Therefore, there is an urgent need to develop bioactive compounds from natural products as alternatives to the very few antibiotics that remain effective. Recently, the efflux mechanism has been identified as the main contributor to antibiotic resistance in bacteria. This study therefore aimed to evaluate tetrandrine (TET), an efflux pump inhibitor (EPI), as a potential antibiotic against MRSA. We investigated the antimicrobial activity of TET against 17 MRSA strains, of which 3 selected strains were studied in further detail using a time-kill assay. When these bacterial al strains (1 x 106 colony-forming units (cfu)/ml) were incubated with TET in a time-kill assay, log-scale bactericidal activity was observed, which lasted for 24 hr. In addition, TET exhibits a synergistic effect when combined with the multi-drug resistance (MDR)-efflux pump substrate ethidium bromide (EtBr). Structure-function studies of the antibiotic activity of TET in combination with EtBr may lead to the discovery of more effective efflux pump inhibitors.
- Subjects
SYNERGETICS; TETRANDRINE; METHICILLIN-resistant staphylococcus aureus; DRUG resistance in microorganisms; ANTIBACTERIAL agents; MULTIDRUG resistance
- Publication
Journal of Toxicological Sciences, 2011, Vol 36, Issue 5, p645
- ISSN
0388-1350
- Publication type
Article
- DOI
10.2131/jts.36.645