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- Title
Azacitidine (5-AzaC)-treatment and mutations in DNA methylase genes affect embryogenic response and expression of the genes that are involved in somatic embryogenesis in Arabidopsis.
- Authors
Grzybkowska, Daria; Morończyk, Joanna; Wójcikowska, Barbara; Gaj, Małgorzata Danuta
- Abstract
Epigenetic processes including DNA methylation play a pivotal role in regulating the genes that control plant development. In contrast to <italic>in planta</italic> development, the contribution of DNA methylation to the morphogenic processes that are induced in vitro are much less recognised. Hence, in the present study, we analysed the impact of DNA methylation on somatic embryogenesis (SE) that was induced in Arabidopsis. The results demonstrated a decrease in the global DNA methylation level during SE that contrasted with the up-regulation of <italic>MET1</italic> and <italic>CMT3</italic> DNA methylases and the down-regulation of DNA demethylases (<italic>ROS1, DME</italic> and <italic>DML2</italic>). Hence, the global DNA methylation level appears not to correlate with the transcriptional activity of the genes encoding DNA methylases/demethylases, thereby implying the complexity of the regulatory mechanism that controls the DNA methylation status of the SE-epigenome. Moreover, distinct changes in the expression level of the SE-regulatory genes were indicated in the 5-AzaC-treated and DNA methylase mutant cultures. Accordingly, a significant repression of the <italic>LEC2, LEC1</italic> and <italic>BBM</italic> genes was found in the 5-AzaC-treated culture that was incapable of SE induction. In contrast, the distinct up-regulation of these genes was observed in the <italic>drm1drm2</italic> and <italic>drm1drm2cmt3</italic> mutant cultures with an improved embryogenic response. The modulated expression of DNA methylase genes and the significantly modified embryogenic response of the <italic>met1</italic> and <italic>drm</italic> mutants imply that both the maintenance and the de novo pathway of DNA methylation are engaged in the regulation of SE in Arabidopsis.
- Publication
Plant Growth Regulation, 2018, Vol 85, Issue 2, p243
- ISSN
0167-6903
- Publication type
Article
- DOI
10.1007/s10725-018-0389-1