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- Title
MicroRNA-148a suppresses epithelial-to-mesenchymal transition by targeting ROCK1 in non-small cell lung cancer cells.
- Authors
Li, Jipeng; Song, Yulan; Wang, Yiping; Luo, Jianping; Yu, Wanjun
- Abstract
Recent studies have implied that miRNAs act as crucial modulators for epithelial-to-mesenchymal transition (EMT). We found that miR-148a is significantly downregulated in non-small cell lung cancer (NSCLC) compared to adjacent non-cancerous lung tissues, and the downregulated miR-148a was significantly associated with lymph-node metastasis. Functional assays demonstrated that miR-148a inhibited EMT in NSCLC cells. Moreover, miR-148a decreased 3′-untranslated region luciferase activity of ROCK1 and ROCK1 protein expression. Knockdown of ROCK1 reversed EMT resembling that of miR-148a overexpression. Furthermore, ROCK1 was widely upregulated in NSCLC, and its mRNA levels were inversely correlated with miR-148a expression. These findings suggest that miR-148a acts as a novel EMT suppressor in NSCLC cells, at least in part by modulation of ROCK1.
- Subjects
MICRORNA; LUNG cancer; LYMPH node cancer; SMALL cell lung cancer; CANCER genetics; CANCER invasiveness
- Publication
Molecular & Cellular Biochemistry, 2013, Vol 380, Issue 1/2, p277
- ISSN
0300-8177
- Publication type
Article
- DOI
10.1007/s11010-013-1682-y