We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Scanning elastic scattering spectroscopy detects metastatic breast cancer in sentinel lymph nodes.
- Authors
Martin R. Austwick; Benjamin Clark; Charles A. Mosse; Kristie Johnson; D. Wayne Chicken; Santosh K. Somasundaram; Katherine W. Calabro; Ying Zhu; Mary Falzon; Gabrijela Kocjan; Tom Fearn; Stephen G. Bown; Irving J. Bigio; Mohammed R. S. Keshtgar
- Abstract
A novel method for rapidly detecting metastatic breast cancer within excised sentinel lymph node(s) of the axilla is presented. Elastic scattering spectroscopy (ESS) is a point-contact technique that collects broadband optical spectra sensitive to absorption and scattering within the tissue. A statistical discrimination algorithm was generated from a training set of nearly 3000 clinical spectra and used to test clinical spectra collected from an independent set of nodes. Freshly excised nodes were bivalved and mounted under a fiber-optic plate. Stepper motors raster-scanned a fiber-optic probe over the plate to interrogate the node’s cut surface, creating a 20×20grid of spectra. These spectra were analyzed to create a map of cancer risk across the node surface. Rules were developed to convert these maps to a prediction for the presence of cancer in the node. Using these analyses, a leave-one-out cross-validation to optimize discrimination parameters on 128 scanned nodes gave a sensitivity of 69 for detection of clinically relevant metastases (71 for macrometastases) and a specificity of 96, comparable to literature results for touch imprint cytology, a standard technique for intraoperative diagnosis. ESS has the advantage of not requiring a pathologist to review the tissue sample.
- Subjects
METASTASIS; BREAST cancer; SENTINEL lymph nodes; ABSORPTION spectra; CYTOLOGY; SPECTRUM analysis; ELASTIC scattering
- Publication
Journal of Biomedical Optics, 2010, Vol 15, Issue 4, p047001
- ISSN
1083-3668
- Publication type
Article
- DOI
10.1117/1.3463005