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- Title
In vitro propagation of an endangered medicinal plant Saussurea involucrata Kar. et Kir.
- Authors
Bin Guo; Min Gao; Chun-Zhao Liu
- Abstract
Abstract??An efficient micropropagation system forSaussurea involucrataKar. et Kir., an endangered Chinese medicinal plant, has been developed. Shoot organogenesis occurred fromS. involucrataleaf explants inoculated on medium with appropriate supplements of plant growth regulators. 66.0% of shoot regeneration frequency and 5.2 shoots per leaf explant were achieved when cultured on a medium containing 10??M 6-benzylaminopurine (BAP) and 2.5??M 1-naphthaleneacetic acid (NAA). Shoot organogenesis was improved further when the leaf explants were pre-incubated at low temperature, and 80.6% of shoot regeneration frequency was recorded with 9.3 shoots per leaf explant at 4?C by 5-day pretreatment period. Up to 87.0% of the regenerated shoots formed complete plantlets on a medium containing 2.5??M indole-3-acetic acid (IAA) within 28 days, and 85.2% of the regenerated plantlets survived and grew vigorously in greenhouse condition. The phytochemical profile of the micropropagated plants was similar to that of wild plants. The regeneration protocol developed in this study provides a basis for germplasm conservation and for further investigation of medicinally active constituents of the elite medicinal plant.
- Subjects
SAUSSUREA; MEDICINAL plants; PLANT regulators; MEDICAL botany
- Publication
Plant Cell Reports, 2007, Vol 26, Issue 3, p261
- ISSN
0721-7714
- Publication type
Article
- DOI
10.1007/s00299-006-0230-6