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- Title
Origin and hierarchy of basal lamina-forming and -non-forming myogenic cells in mouse skeletal muscle in relation to adhesive capacity and Pax7 expression in vitro.
- Authors
Tamaki, Tetsuro; Tono, Kayoko; Uchiyama, Yoshiyasu; Okada, Yoshinori; Masuda, Maki; Soeda, Shuichi; Nitta, Masahiro; Akatsuka, Akira
- Abstract
a novel approach to distinguish skeletal myogenic cell populations, basal lamina (BL) formation of myogenic cells was examined in the mouse compensatory enlarged plantaris muscles in vivo and in fiber-bundle cultures in vitro. MyoD myogenic cells located inside the regenerative muscle fiber BL were laminin but interstitial MyoD cells were laminin. This was also confirmed by electron microscopy as structural BL formation. Similar trends were observed in the fiber-bundle cultures including satellite cells and interstitial myogenic cells and laminin myogenic cells predominantly showed non-adhesive (non-Ad) behavior with Pax7, whereas laminin cells were adhesive (Ad) with Pax7. Moreover, non-Ad/laminin and Ad/laminin myotubes were also observed and the former type showed spontaneous contractions, while the latter type did not. The origin and hierarchy of Ad/Pax7/laminin and non-Ad/Pax7/laminin myogenic cells were also examined using skeletal muscle interstitium-derived CD34/45 (Sk-34) and CD34/45 (Sk-DN) multipotent stem cells, which were composed of non-committed myogenic cells with a few (<1%) Pax7 cells in the Sk-DN cells at fresh isolation. Both cell types were separated by Ad/non-Ad capacity in repetitive culture. As expected, both Ad/Pax7/laminin and non-Ad/Pax7/laminin myogenic cells consistently appeared in the Ad and non-Ad cell culture. However, Ad/Pax7/laminin cells were repeatedly detected in the non-Ad cell culture, while the opposite phenomenon did not occur. This indicates that the source of non-Ad/ Pax7/laminin myogenic cells was present in the Sk-34 and Sk-DN stem cells and they were able to produce Ad/ Pax7/ laminin myogenic cells during myogenesis as primary myoblasts and situated hierarchically upstream of the latter cells.
- Subjects
BASAL lamina; MYOBLASTS; MUSCULOSKELETAL system; LABORATORY mice; CELL adhesion; REVERSE transcriptase polymerase chain reaction; MYOGENESIS
- Publication
Cell & Tissue Research, 2011, Vol 344, Issue 1, p147
- ISSN
0302-766X
- Publication type
Article
- DOI
10.1007/s00441-010-1127-9