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- Title
Characterization of an atypical, thermostable, organic solvent- and acid-tolerant 2′-deoxyribosyltransferase from Chroococcidiopsis thermalis.
- Authors
Del Arco, Jon; Sánchez-Murcia, Pedro Alejandro; Mancheño, José Miguel; Gago, Federico; Fernández-Lucas, Jesús
- Abstract
In our search for thermophilic and acid-tolerant nucleoside 2′-deoxyribosyltransferases (NDTs), we found a good candidate in an enzyme encoded by Chroococcidiopsis thermalis PCC 7203 (CtNDT). Biophysical and biochemical characterization revealed CtNDT as a homotetramer endowed with good activity and stability at both high temperatures (50-100 °C) and a wide range of pH values (from 3 to 7). CtNDT recognizes purine bases and their corresponding 2′-deoxynucleosides but is also proficient using cytosine and 2′-deoxycytidine as substrates. These unusual features preclude the strict classification of CtNDT as either a type I or a type II NDT and further suggest that this simple subdivision may need to be updated in the future. Our findings also hint at a possible link between oligomeric state and NDT’s substrate specificity. Interestingly from a practical perspective, CtNDT displays high activity (80-100%) in the presence of several water-miscible co-solvents in a proportion of up to 20% and was successfully employed in the enzymatic production of several therapeutic nucleosides such as didanosine, vidarabine, and cytarabine.
- Subjects
NUCLEOSIDE deoxyribosyltransferase; CHROOCOCCACEAE; CYANOBACTERIA biotechnology; DEOXYCYTIDINE; CYTOSINE; DIDANOSINE (Drug); CYTARABINE
- Publication
Applied Microbiology & Biotechnology, 2018, Vol 102, Issue 16, p6947
- ISSN
0175-7598
- Publication type
Article
- DOI
10.1007/s00253-018-9134-y