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- Title
Effect of Matrix Metalloproteinase Inhibitors on Rat Embryo Development in vitro.
- Authors
Del Bigio, Marc R.; Seyoum, Girma
- Abstract
Matrix metalloproteinases appear to play a role in cell migration and tissue remodeling and are postulated to be important in the development of embryos. We hypothesized that inhibition of these proteinases with the synthetic inhibitor batimastat or with endogenous tissue inhibitor of metalloproteinases (TIMP) would alter in vitro development of day 9 rat embryos. Batimastat had a dose-related effect on embryo growth and viability following 48 h in culture, with significant inhibition at 1.0 μM concentration. Dying cells were observed in many organs of living embryos. TIMP-2 at 3.0 μg/ml had a similar effect on embryo growth. TIMP-1 alone or used in combination with TIMP-2 had no obvious effect. No focal malformations were observed. The effective concentrations are comparable to those which inhibit enzyme activity in cell culture. We conclude that matrix metalloproteinases are probably important in mammalian embryo development.
- Subjects
GROWTH; METALLOPROTEINASES; PROTEINASES; EMBRYOS; RATS
- Publication
Cells Tissues Organs, 1999, Vol 165, Issue 2, p67
- ISSN
1422-6405
- Publication type
Article
- DOI
10.1159/000016676