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- Title
Nucleotide sequence of a macronuclear gene for actin in Oxytricha fallax.
- Authors
Kaine, Brian P.; Spear, Brian B.
- Abstract
The hypotrichous ciliated protozoan Oxytricha fallax possesses a mechanism for processing its genome into a collection of small, gene-sized, transcriptionally active sections. Macronuclear DNA is arranged as short achromosomal sections, 0.5-22 kilo-base pairs (kbp) long, while micronuclear DNA has a typical chromosomal organization1,2. Macronuclear DNA is derived from micronuclear DNA by a process of polytene chromosome fragmentation with a resultant decrease in DNA sequence complexity3,4. The isolation of intact macronuclear DNA sections enables the study of specific structural units of genetic material that occur naturally in the organism. We have previously described5 the construction of a recombinant plasmid, pOfACT (1.6), which contains an intact 1.6-kb length of macronuclear DNA (Fig. 1) homologous to a yeast actin gene6. We now present the complete nucleotide sequence of the cloned DNA molecule and the derived sequence of the actin protein which it encodes. As the sequence is that of a complete, naturally occurring DNA molecule, it shows all cis-active regulatory regions in addition to the coding region. The actin protein encoded differs considerably from other known actins.
- Publication
Nature, 1982, Vol 295, Issue 5848, p430
- ISSN
0028-0836
- Publication type
Article
- DOI
10.1038/295430a0