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- Title
Isolation of I-A subregion-like molecules from subhuman primates and man.
- Authors
Goyert, Sanna M.; Silver, Jack
- Abstract
Immune response-associated (Ia) molecules play a major part in cellular interactions that initiate an immune response. Two families of Ia molecules, determined by the I-A and I-E subregions of the murine major histocompatibility complex (MHC), have been isolated and characterized in mice1. I-A and I-E molecules each consist of two polypeptide chains (molecular weights (Mrs) of 26,000 and 35,000 for I-A and 24,000 and 32,000 for I-E) that are noncovalently associated2. Their large subunits are designated Aα and Eα, respectively, and their small subunits Aβ and Eβ. Despite the similarity in their subunit composition, the I-A and I-E molecules are structurally distinct according to peptide mapping and partial N-terminal sequence analysis3,4. Although the structural homologue of murine I-E molecules has been identified in man and is known as HLA-DR5, the I-A equivalent has never been described. In an attempt to identify the human analogue of the murine I-A molecule, murine monoclonal antibodies were produced after immunization with a mixture of three putative human cell lines. We report here that one of these antibodies reacts with I-A-like molecules from two cell lines, GM3158, which after karyotype analysis was shown to have originated not from man but from a marmoset, and GM3163, a cell line of human origin.
- Publication
Nature, 1981, Vol 294, Issue 5838, p266
- ISSN
0028-0836
- Publication type
Article
- DOI
10.1038/294266a0