We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Identification of the nucleoli of Giardia lamblia with TEM and CFM.
- Authors
Xi Feng Tian; Zhi Hong Yang; Haie Shen; Adam, R. D.; Si Qi Lu
- Abstract
Giardia species are flagellated parasites of vertebrates and belong to the diplomonads, most of which have two nuclei. These organisms were classified among the earliest branching eukaryotes on the basis of small subunit rDNA sequences and their lack of many canonical organelles. However, some of these organelles have subsequently been identified in rudimentary form, such as Golgi that become apparent during encystation. One of the "missing" organelles has been the nucleolus, the site of rRNA synthesis, since it was not identified in earlier ultrastructural studies. In the current study, we visualized in-vitro-grown Giardia lamblia trophozoites by transmission electron microscopy and laser scanning confocal microscopy. We found that each of the two nuclei contains a single small and deeply stained granular nucleolus, thus demonstrating that Giardia does indeed have nucleoli.
- Subjects
GIARDIA; GIARDIASIS; PARASITES; VERTEBRATES; RECOMBINANT DNA; TRANSMISSION electron microscopy
- Publication
Parasitology Research, 2010, Vol 106, Issue 4, p789
- ISSN
0932-0113
- Publication type
Article
- DOI
10.1007/s00436-009-1715-3