We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Colony growth of Philippine isolates of Blastocystis hominis in simplified, soft agar medium.
- Authors
Ezra Valido
- Abstract
Abstract  The agar-cloning technique of Blastocystis hominis has been observed in both solid and semisolid agar using Iscoveâs modified Dulbeccoâs medium. In this study, Philippine isolates of B. hominis were grown by pour-plate method in semisolid agar using Lockeâs solution. Inoculated plates contained 0.7% agar, 10% horse serum, and 0.1% sodium thioglycollate. Plates were incubated at 37C in a microaerophilic jar for 7â10 days. Biconvex disk-shaped colonies were seen abound at the bottom half of the medium. Colonies growing at the agarâglass interface were flat and consisted of thin layers of cells. From these colonies, large amoeboid cells were frequently seen on the periphery, whereas smaller cells were concentrated at the core. Analysis of the SSU rDNA genetically established the identity of the clones to be B. hominis. This is the first report on agar cloning of Blastocystis in a compound medium.
- Subjects
POLYSACCHARIDES; ALGAE; CLONING; GENETIC engineering
- Publication
Parasitology Research, 2007, Vol 101, Issue 1, p213
- ISSN
0932-0113
- Publication type
Article
- DOI
10.1007/s00436-006-0456-9