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- Title
SV40 large T shares an antigenic determinant with a cellular protein of molecular weight 68,000.
- Authors
Lane, D. P.; Hoeffler, W. K.
- Abstract
The large T protein coded by the early region of simian virus 40 (SV40) is involved in the induction and maintenance of cell transformation1. It is not clear which, properties of T are important in causing the transformation, since the protein is multifunctional2-9. To clarify the action of T proteins in transformation, we have prepared several monoclonal antibodies directed against different regions of the T molecule. One of these antibodies, DL 3C4, recognizes an antigenic determinant on T that is also present on a host cell protein of molecular weight 68,000. This cross-reactive 68K protein is located within the nucleus of all mammalian cell types examined and has a characteristic granular distribution as shown by immuno-fluorescence. The antigenic determinant recognized by DL 3C4 is resistant to denaturation. Studies on Adeno-SV40 hybrid viruses show that the antigenic site on T is coded for by sequences located between 0.44 and 0.29 on the SV40 map.
- Publication
Nature, 1980, Vol 288, Issue 5787, p167
- ISSN
0028-0836
- Publication type
Article
- DOI
10.1038/288167a0