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- Title
FGT-1 is the major glucose transporter in C. elegans and is central to aging pathways.
- Authors
Ying FENG; WILLIAMS, Barnabas G.; KOUMANOV, Françoise; WOLSTENHOLME, Adrian J.; HOLMAN, Geoffrey D.
- Abstract
Caenorhabditis elegans is widely used as amodel for investigation of the relationships between aging, nutrient restriction and signalling via the DAF-2 (abnormal dauer formation 2) receptor for insulin-like peptides and AGE-1 [ageing alteration 1; orthologue of PI3K (phosphoinositide 3-kinase)], but the identity of the glucose transporters that may link these processes is unknown. We unexpectedly find that of the eight putative GLUT (glucose transporter)-like genes only the two splice variants of one gene have a glucose transport function in an oocyte expression system. We have named this gene fgt-1 (facilitated glucose transporter, isoform 1). We show that knockdown of fgt- 1 RNA leads to loss of glucose transport and reduced glucose metabolism in wild-type worms. The FGT-1 glucose transporters of C. elegans thus play a key role in glucose energy supply to C. elegans. Importantly, knockdown of fgt-1 leads to an extension of lifespan equivalent, but not additive, to that observed in daf-2 and age-1 mutant worms. The results of the present study are consistent with DAF-2 and AGE-1 signalling stimulating glucose transport in C. elegans and this process being associated with the longevity phenotype in daf-2 and age-1 mutant worms. We propose that fgt-1 constitutes a common axis for the lifespan extending effects of nutrient restriction and reduced insulin-like peptide signalling.
- Subjects
GLUCOSE transporters; CAENORHABDITIS elegans; AGING; CELLULAR signal transduction; DEVELOPMENTAL biology; PROINSULIN
- Publication
Biochemical Journal, 2013, Vol 456, Issue 2, p219
- ISSN
0264-6021
- Publication type
Article
- DOI
10.1042/BJ20131101