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- Title
Changes in morphology and inward rectifier currents in human atrial myocytes depend on culture conditions.
- Authors
Himmel, Herbert M.; Pietsch, Martina; Streller, Uwe; Graf, Eva M.; Ravens, Ursula
- Abstract
Human atrial myocytes were cultured under systematically varied conditions in order to obtain stable cells for future gene manipulation. Transient (Ito) and sustained outward current (Iso), and voltage- and muscarinic receptor-activated inward rectifier K+ currents (IK1, IK,ACh) were measured in freshly isolated cells and after 5 days in culture. Myocytes were grown on polylysin or laminin in medium with or without 10 % serum (medium+S, medium-S). Cultured myocytes dedifferentiated to a greater extent in medium+S than medium-S, but independent of the chemical nature of the adherence surface. Apparent surface area increased in medium+S, whereas membrane capacitance declined under all culture conditions. Ito of myocytes cultured in medium-S was increased. Myocytes grown on polylysin and laminin exhibited reduced IK1 current density. Under all culture conditions, IK,ACh was attenuated with carbachol but hardly affected with sphingosine-1-phosphate as agonists. In conclusion, morphological and electrophysiological changes depended on serum in the culture medium rather than on adherence surface being coated with laminin or polylysin.
- Subjects
MUSCLE cells; SERUM; CELLS; BLOOD plasma; SPHINGOSINE; ETHANOLAMINES
- Publication
Basic Research in Cardiology, 2002, Vol 97, Issue 6, p434
- ISSN
0300-8428
- Publication type
Article
- DOI
10.1007/s00395-002-0375-6