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- Title
Genome-wide analysis and expression profiling of the <italic>HMA</italic> gene family in <italic>Brassica napus</italic> under cd stress.
- Authors
Li, Nannan; Xiao, Hua; Sun, Juanjuan; Wang, Shufeng; Wang, Jingchao; Chang, Peng; Zhou, Xinbin; Lei, Bo; Lu, Kun; Luo, Feng; Shi, Xiaojun; Li, Jiana
- Abstract
Background and aims: <italic>Brassica napus</italic> (<italic>B. napus</italic>), one of the most important oil crop species, is highly tolerant to and accumulates high amounts of cadmium (Cd). Many iron transporters in plants have been identified to be Cd transporters. For example, some members of the heavy metal P1B-ATPase transporter family are responsible for Cd translocation in various plant species and play a vital role in Cd detoxification. However, the Cd translocation mechanism in <italic>B. napus</italic> and the characterization of the heavy metal ATPase (HMA) in <italic>B. napus</italic> remain unknown.Methods: <italic>B. napus</italic> plants were treated with 50 μM or 200 μM Cd in soil for 30 days during the initial flowering stage. The dry weight of the plants and the Cd contents within their various tissues were then measured, after which the RNA in the leaves was extracted for transcriptomic analysis and subsequent quantitative real-time PCR (qRT-PCR) assays. After all the significantly regulated iron transporters were screened in response to Cd stress, the <italic>BnHMA</italic> gene family was identified and shown to link <italic>BnHMA</italic> genes with Cd translocation in the leaves of <italic>B. napus.</italic>Results: The transcriptomic analysis of <italic>B. napus</italic> leaves in response to Cd treatment revealed that several <italic>HMA</italic> genes (<italic>BnHMA2;2</italic>, <italic>BnHMA2;3</italic> and <italic>BnHMA2;5</italic>) respond to Cd stress. We further examined the whole <italic>HMA</italic> family in <italic>B. napus</italic>; 31 <italic>BnHMA</italic> genes were subsequently identified. Their expression levels in different tissues and stages as well as their phylogenetic tree, gene structure, chromosomal location, conserved motifs, 3D models and subcellular localization were analyzed. The results showed that these <italic>HMA</italic> genes exhibit typical characteristics of <italic>HMA</italic> genes. In addition, the qRT-PCR results showed that the <italic>BnHMA2;3</italic> expression levels in the <italic>B. napus</italic> plants treated with 50 μM or 200 μM Cd were seven- and ninefold greater than those under Cd-free conditions, respectively. Additional yeast experiment assays verified that <italic>BnHMA2;3</italic> can transport Cd.Conclusion: <italic>BnHMA2;3</italic> may play an important role in Cd translocation in the leaves of <italic>B. napus.</italic> The results of this study may provide direction and useful information for increased understanding of the Cd stress-response mechanism.
- Subjects
BRASSICA; PLANT genomes; EFFECT of cadmium on plants; EFFECT of stress on plants; PLANT translocation
- Publication
Plant & Soil, 2018, Vol 426, Issue 1/2, p365
- ISSN
0032-079X
- Publication type
Article
- DOI
10.1007/s11104-018-3637-2