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- Title
Regulation of BTG3 by microRNA-20b-5p in non-small cell lung cancer.
- Authors
Peng, Lijun; Li, Shaobin; Li, Yuchan; Wan, Minghui; Fang, Xisheng; Zhao, Yongxin; Zuo, Wei; Long, De; Xuan, Yiwen
- Abstract
The present study aimed to evaluate microRNA- 20b-5p (miR-20b-5p) expression in non-small cell lung cancer (NSCLC), and investigate the effects of miR-20b-5p expression on NSCLC cell proliferation and migration. Reverse transcription-quantitative polymerase chain reaction was performed to measure the expression level of miR-20b-5p in NSCLC tissues and cell lines. Cell Counting Kit-8 and wound healing assays were used to measure cell proliferation and migration. A dual-luciferase reporter assay was performed to validate B-cell translocation gene 3 (BTG3) as a target of miR-20b-5p. It was identified that the expression level of miR-20b-5p is elevated in NSCLC tissues and cell lines. miR-20b-5p overexpression was revealed to promote NSCLC cell proliferation and migration. Furthermore, BTG3 was identified as a direct target of miR-20b-5p, and BTG3 overexpression reversed a miR-20b-5p mimic-induced increase in cell proliferation and migration. In summary, the present study revealed that miR-20b-5p promotes NSCLC cell proliferation and migration by targeting BTG3, which may assist with the development of a novel therapeutic target for the treatment of NSCLC.
- Subjects
NON-small-cell lung carcinoma; CELL migration; CELL proliferation; POLYMERASE chain reaction
- Publication
Oncology Letters, 2019, Vol 18, Issue 1, p137
- ISSN
1792-1074
- Publication type
Article
- DOI
10.3892/ol.2019.10333