We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Identification of potential vaccine targets for COVID‐19 by combining single‐cell and bulk TCR sequencing.
- Authors
Wang, Pingping; Xu, Zhaochun; Zhou, Wenyang; Jin, Xiyun; Xu, Chang; Luo, Meng; Ma, Kexin; Cao, Huimin; Huang, Yan; Lin, Xiaoyu; Pang, Fenglan; Li, Yiqun; Jiang, Qinghua
- Abstract
Identification of potential vaccine targets for COVID-19 by combining single-cell and bulk TCR sequencing PBMCs were isolated for subsequent data generation: (1) single-cell transcriptome sequencing, (2) single-cell TCR sequencing, (3) deep TCR repertoire sequencing, and (4) HLA genotyping (Figure 1A and Table S1). Overall, we totally obtained single cell gene expression data from 26 223 T cells, single cell paired TCRs from 27 467 T cells and hypervariable regions of immune receptors from 4.9 million TCR clones (Table S2). Here, we use scRNA-seq, scTCR-seq, deep TCR-seq, and HLA-genotyping to decode the matching T cell phenotype and antigenic epitopes of 16 early-recovery COVID-19 patients in the context of human HLA haplotypes.
- Subjects
COVID-19 vaccines; REGULATORY T cells; T cell receptors; COVID-19; CYTOTOXIC T cells; IMMUNOGLOBULIN producing cells; COVID-19 pandemic
- Publication
Clinical & Translational Medicine, 2021, Vol 11, Issue 5, p1
- ISSN
2001-1326
- Publication type
Article
- DOI
10.1002/ctm2.430