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- Title
Estudo in vitro do potencial citotóxico da Annona muricata L.
- Authors
Firmino Silva, Egidi Mayara; de Castro Nascimento, Renata Barreto; Barreto, Francisco Stefânio; de Moraes Filho, Manoel Odorico; Souza Griz, Samara de Almeida; Feitosa dos Santos, Aldenir; Cerqueira Mousinho, Kristiana
- Abstract
This study aims to evaluate the cytotoxic activity of the ethanol extract of the stem bark (AMC) and leaf (MFA) of Annona muricata Linn. To conduct this study was initially verified the activity of the ethanol extract at concentrations of 1000, 800, 600, 200 and 100 µgmL-1 for MFA and concentrations of 200, 150, 100, 50, 10 µgmL-1 for AMC through assay of Artemia salina Leach which is considered a preliminary study on bioassay of extracts with strong biological activity. Subsequently, the cytotoxicity assay was performed using the MTT (method 3 - (4,5 -dimethylthiazol- 2YL ) -2,5- diphenyl bromide in tetrazolina lines SF- 295 (glioblastoma - human), OVCAR -8 (ovarian) HCT -116 (colon) and HL -60 (promyelocytic leukemia). The extracts were tested at a concentration of 50 µg/mL for the single concentration cytotoxicity test to determine the presence or absence of activity. For determination of inhibitory concentration (IC50), all samples were tested in serial concentrations ranging from 0, 09 to 50 µg/mL using 2 as the dilution factor. The present study, both samples used by testing Artemia salina Leach had higher LC50 80 µgmL-1. Sheet presented LC50 = 324, 07 µgmL-1 and stem bark showed LC50 = 196, 04 µgmL-1. Already through MTT inhibitory concentration values (IC50) ranged from 12, 81 to 22,65 µg/mL for MFA and from 0,09 to <5µg/ mL for AMC, evaluated against various tumor cell lines. Results obtained for the bark and leaves of A. muricata, evaluated in this work through toxicity bioassays with Artemia salina L and tumor cells SF-295, OVCAR-8, HCT-116 and HL-60, we can verify the toxic potential of both samples, especially more hulls which had a higher cytotoxic potential. Due to this fact, further experiments should be conducted to further investigate the antitumor potential of the stem bark of A. muricata.
- Publication
Revista de Ciências Farmacêuticas Básica e Aplicada, 2015, Vol 36, Issue 2, p277
- ISSN
1808-4532
- Publication type
Article