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- Title
Abnormal N-Glycosylation of Human Lens Epithelial Cells in Type-2 Diabetes May Contribute to Cataract Progression.
- Authors
Ramos-Martínez, Ivan; Vivanco-Rojas, Oscar; Juárez-Domínguez, Brenda; Hernández-Zimbrón, Luis; Paz, Lenin Ochoa-de la; Quiroz-Mercado, Hugo; Ramírez-Hernández, Eleazar; Gulias-Cañizo, Rosario; Zenteno, Edgar
- Abstract
Purpose: In order to better understand cataract development, we analyzed the glycosylation profile of human lens epithelial cells (HLECs) from anterior lens capsules of type 2 diabetes mellitus (T2DM) and non-diabetic (ND) patients undergoing routine cataract surgery. Setting: Research Department of the Asociación para Evitar la Ceguera, Hospital "Dr. Luis Sánchez Bulnes", Mexico. Design: Experimental study. Methods: Evaluation of anterior lens capsules from T2DM and ND patients undergoing phacoemulsification and free from other ocular diseases. Results: Hematoxylin-eosin staining revealed HLECs alterations in T2DM samples. From lectins with different sugar specificities used, concanavalin A showed significant differences, labeling homogeneously both in the cytoplasm and in cell membranes in ND capsules, while in T2DM capsules, in addition to membrane and cytoplasm labeling, there were perinuclear vesicles with high concanavalin A labeling. Two-dimensional gel electrophoresis showed that T2DM patients have a ∼ 65-kDa spot with an isoelectric point of 5.5 with a higher density compared to ND capsules, and liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis showed 62% homology with type-1 cytokeratin. Immunohistochemistry using anti-pan cytokeratin antibody revealed co-localization with concanavalin A, and a lectin blot revealed with concanavalin A showed a band of ∼ 65 kDa, a molecular weight that corresponds to human type 1 cytokeratin. Conclusion: These results suggest that over-expression of N-glycosidically linked human type 1 cytokeratin may induce capsule disruption and affect selective permeability, allowing the entry of different molecules to the lens that facilitate cataract progression.
- Subjects
MEXICO; GEL electrophoresis; TYPE 2 diabetes; EPITHELIAL cells; PHACOEMULSIFICATION; CONCANAVALIN A; LIQUID chromatography-mass spectrometry; CATARACT
- Publication
Clinical Ophthalmology, 2021, Vol 15, p1365
- ISSN
1177-5467
- Publication type
Article
- DOI
10.2147/OPTH.S300242