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- Title
Evaluation of antitumor activity of a TGF-beta receptor I inhibitor (SD-208) on human colon adenocarcinoma.
- Authors
Akbari, Abolfazl; Amanpour, Saeid; Muhammadnejad, Samad; Hossein Ghahremani, Mohammad; Hamidollah Ghaffari, Seyed; Reza Dehpour, Ahmad; Reza Mobini, Gholam; Shidfar, Fatemeh; Abastabar, Mahdi; Khoshzaban, Ahad; Faghihloo, Ebrahim; Karimi, Abbas; Heidari, Mansour
- Abstract
Background: Transforming growth factor-β (TGF-β) pathway is involved in primary tumor progression and in promoting metastasis in a considerable proportion of human cancers such as colorectal cancer (CRC). Therefore, blockage of TGF-β pathway signaling via an inhibitor could be a valuable tool in CRC treatment. To evaluate the efficacy of systemic targeting of the TGF-β pathway for therapeutic effects on CRC, we investigated the effects of a TGβRI (TGF-β receptor 1) or TβRI kinase inhibitor, SD-208, on SW-48, colon adenocarcinoma cells. In this work, in vitro cell proliferation was studied by Methyl thiazolyl tetrazolium (MTT) and Bromo-2′-deoxyuridine (BrdU) assays. Also, the histopathological and immunohistochemical evaluations were conducted by hematoxylin and eosin, and Ki-67 and CD34 markers are staining, respectively. Methods: To evaluate the efficacy of systemic targeting of the TGF-β pathway for therapeutic effects on CRC, we investigated the effects of a TGβRI (TGF-β receptor 1) or TβRI kinase inhibitor, SD-208, on SW-48, colon adenocarcinoma cells. In this work, in vitro cell proliferation was studied by Methyl thiazolyl tetrazolium (MTT) and Bromo-2′-deoxyuridine (BrdU) assays. Also, the histopathological and immunohistochemical evaluations were conducted by hematoxylin and eosin, and Ki-67 and CD34 markers are staining, respectively. Results: Our results showed no significant reduction in cell proliferation and vessel formation (170 ± 70 and 165 ± 70, P > 0.05) in treated SW-48 cells with SD-208 compared to controls. Conclusion: Our data suggested that SD-208 could not significantly reduce tumor growth and angiogenesis in human colorectal cancer model at least using SW-48 cells.
- Subjects
STAINS &; staining (Microscopy); IMMUNOHISTOCHEMISTRY; ADENOCARCINOMA; ANALYSIS of variance; ANIMAL experimentation; BIOLOGICAL assay; BIOPHYSICS; CELL culture; CELLULAR signal transduction; COLON tumors; ENZYME-linked immunosorbent assay; GROWTH factors; HISTOLOGICAL techniques; RESEARCH methodology; MICE; STATISTICS; T-test (Statistics); DATA analysis; PROTEIN kinase inhibitors; DESCRIPTIVE statistics; IN vitro studies
- Publication
DARU: Journal of Pharmaceutical Sciences, 2014, Vol 22, p47
- ISSN
1560-8115
- Publication type
Article
- DOI
10.1186/2008-2231-22-47