We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
The role of store-operated calcium channels in early brain injury after subarachnoid hemorrhage.
- Authors
ZHANG Ming; WU Yuan; SONG Jin-ning; ZHAO Yong-lin; FU Zhou-feng; MA Xu-dong; ZHAI Hai-cheng
- Abstract
Objective To investigate the possible role of store-operated calcium channels (SOCC) in early brain injury (EBI) after subarachnoid hemorrhage (SAH) and to explore the relationship between SOCC and calcium overload induced delayed neuronal death (DND) after SAH. Methods Rabbits models of SAH were established using the single-blood injection method via the cisterna magna. SAH intervention group was established by intracerebroventricular injection of 2-aminoethyldiphenyl borate (2-APB). Intracellular calcium concentration was detected by Fura-2AM. The dynamic expression of SOCC was determined by Western blot. The apoptosis of cortical neurons was detected by TUNEL. One-way ANOVA was used to compare between experimental, normal control and sham-operation groups. Results The protein expressions of STIM1 and ORAI1 in the cortex increased continuously after SAH and reached the peak at day 3; intracellular calcium concentraron also reached the peak. After injection of SOCC blocker, the protein expressions of STIM1 and ORAI1 were inhibíted and intracellular calcium concentration decreased. Meanwhile, the scores of neurological functions.
- Subjects
CALCIUM channels; BRAIN injuries; SUBARACHNOID hemorrhage; APOPTOSIS; WESTERN immunoblotting; COMPARATIVE studies
- Publication
Journal of Xi'an Jiaotong University (Medical Sciences), 2013, Vol 34, Issue 6, p710
- ISSN
1671-8259
- Publication type
Article
- DOI
10.7652/jdyxb201306003