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- Title
High-affinity binders selected from designed ankyrin repeat protein libraries.
- Authors
Binz, H Kaspar; Amstutz, Patrick; Kohl, Andreas; Stumpp, Michael T.; Briand, Christophe; Forrer, Patrik; Grütter, Markus G.; Plückthun, Andreas
- Abstract
We report here the evolution of ankyrin repeat (AR) proteins <em>in vitro</em> for specific, high-affinity target binding. Using a consensus design strategy, we generated combinatorial libraries of AR proteins of varying repeat numbers with diversified binding surfaces. Libraries of two and three repeats, flanked by `capping repeats,' were used in ribosome-display selections against maltose binding protein (MBP) and two eukaryotic kinases. We rapidly enriched target-specific binders with affinities in the low nanomolar range and determined the crystal structure of one of the selected AR proteins in complex with MBP at 2.3 Å resolution. The interaction relies on the randomized positions of the designed AR protein and is comparable to natural, heterodimeric protein-protein interactions. Thus, our AR protein libraries are valuable sources for binding molecules and, because of the very favorable biophysical properties of the designed AR proteins, an attractive alternative to antibody libraries.
- Subjects
MALTOSE; CARRIER proteins; IMMUNOGLOBULINS; BIOLOGICAL transport; DISACCHARIDES; RIBOSOMES
- Publication
Nature Biotechnology, 2004, Vol 22, Issue 5, p575
- ISSN
1087-0156
- Publication type
Article
- DOI
10.1038/nbt962