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- Title
Synthetic muscle promoters: activities exceeding naturally occurring regulatory sequences.
- Authors
Li, Xuyang; Eastman, Eric M.; Schwartz, Robert J.; Draghia-Akli, Ruxandra
- Abstract
Relatively low levels of expression from naturally occurring promoters have limited the use of muscle as a gene therapy target. Myogenic restricted gene promoters display complex organization usually involving combinations of several myogenic regulatory elements. By random assembly of E-box, MEF-2, TEF-1, and SRE sites into synthetic promoter recombinant libraries, and screening of hundreds of individual clones for transcriptional activity in vitro and in vivo, several artificial promoters were isolated whose transcriptional potencies greatly exceed those of natural myogenic and viral gene promoters.
- Subjects
PROMOTERS (Genetics); GENE therapy
- Publication
Nature Biotechnology, 1999, Vol 17, Issue 3, p241
- ISSN
1087-0156
- Publication type
Article
- DOI
10.1038/6981