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- Title
The Expression of Tumor Necrosis Factor-α (TNF-α) by the Intrathecal Injection of Lipopolysaccharide in the Rat Spinal Cord.
- Authors
Aiguo Shen; Qin Shen; Hai-ou Liu; Linlin Sun; Yonghua Liu; Jianping Chen; Junling Yang; Yuhong Ji
- Abstract
Abstract The proinflammatory and lipopolysaccharide (LPS)-inducible cytokine tumor necrosis factor α (TNF-α) has been shown to enhance primary sensory nociceptive signaling. However, the precise cellular site of TNF-α synthesis is still a matter of controversy. Therefore, we focused our study on TNF-α protein synthesis and expression patterns in spinal dorsal horn of naives and rats under intrathecal challenge with LPS. The enzyme-linked immunosorbent (ELISA) assay showed that the protein level of TNF-α reached peak at 8 h. Double immunofluorescence revealed that LPS-induced expression of TNF-α exclusively located in a subpopulation of microglia, which increased at 8 h in the rat spinal dorsal horn (the injected side). Positive staining of TNF receptor 1 (TNFR1) were also found in microglia. These observations have demonstrated the production of this proinflammatory cytokine by central nerve glia especially microglia. Synthesized TNF-α might directly act on microglia via TNFR1, but the inherent mechanisms remain unknown. Further studies are needed to confirm the pathogenic role of tumor necrosis factor in the early stage of inflammation.
- Subjects
TUMOR necrosis factors; GENE expression; CELL communication; ENDOTOXINS; SPINAL cord; SPINAL injections; PROTEIN synthesis; LABORATORY rats; INTRATHECAL injections
- Publication
Neurochemical Research, 2009, Vol 34, Issue 2, p333
- ISSN
0364-3190
- Publication type
Article
- DOI
10.1007/s11064-008-9780-z