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- Title
Antiviral Potential of Ivermectin against Peste des Petits Ruminants Virus (PPRV).
- Authors
Afzal, Saba; Raza, Sohail; Rabbani, Masood; Firyal, Sehrish; Altaf, Imran; Naeem, Zahra
- Abstract
This study was conducted to find the antiviral potential of ivermectin against Peste des Petits Ruminants Virus (PPRV) in vitro. Firstly, cell cytotoxicity of ivermectin in the Vero cells was determined using MTT assay. The antiviral activity of ivermectin was determined using viral inhibition assays and median tissue culture infectious dose (TCID50). The results showed that 2.5 µM concentration of the ivermectin is nontoxic to the Vero cells. At this concentration, PPRV titre was significantly reduced (p < 0.001) by two log to 103.0 TCID50/0.1 mL as compared to virus control 105.5 TCID50/0.1 mL. Moreover, the ivermectin exposure after the viral attachment and entry steps able to reduce virus titre more as compare to viral attachment and entry steps that predicts the possible mechanism of the drug. Therefore, our study first time demonstrated the antiviral potential of ivermectin against PPRV in vitro and its future potential for its use as anti-PPRV therapeutics.
- Subjects
IVERMECTIN; PESTE des petits ruminants; ANTIVIRAL agents; TISSUE culture
- Publication
Pakistan Journal of Zoology, 2021, Vol 53, Issue 4, p1575
- ISSN
0030-9923
- Publication type
Article
- DOI
10.17582/journal.pjz/20200704060712