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- Title
Evaluation of the stability of an SR398/GroES chaperonin complex.
- Authors
Ishino, So; Kawata, Yasushi; Ikegami, Takahisa; Matsuzaki, Katsumi; Hoshino, Masaru
- Abstract
The stability of an SR398/GroES chaperonin complex was examined. As was expected, based on the finding of previous studies, the SR398/GroES complex was extremely stable in the presence of an excess amount of free adenosine 5′-[γ-thio]triphosphate (ATPγS) or adenosine 5′-(β,γ-imido)triphosphate (AMPPNP). However, the complex was not stable in the absence of nucleotides. These results indicate that ATPγS and AMPPNP repeatedly associated to and dissociated from the complex in a non-cooperative manner. This nucleotide exchange did not induce the dissociation of GroES and substrate from SR398, suggesting the importance of the cooperative dissociation of nucleotides from the cis-ring to release GroES and substrate proteins in the GroEL/GroES reaction cycle.
- Subjects
MOLECULAR chaperones; ADENOSINE triphosphate; NUCLEOTIDES; NUCLEOTIDE exchange factors; DISSOCIATION (Chemistry)
- Publication
Journal of Biochemistry, 2014, Vol 155, Issue 5, p295
- ISSN
0021-924X
- Publication type
Article
- DOI
10.1093/jb/mvu009