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- Title
A new technique for analyzing trace element uptake by human enamel.
- Authors
Yoshiki FUNATO; Yasuhiro MATSUDA; Katsushi OKUYAMA; Hiroko YAMAMOTO; Hisanori KOMATSU; Hidehiko SANO
- Abstract
Fluorine (F) and strontium (Sr) are key elements in the de- and remineralization of teeth. To quantitatively analyze the distribution of F and Sr, micro-particle-induced gamma/X-ray emission (PIGE/PIXE) technique was used. The cavities were prepared and filled with the fluoride- and Sr-containing restorative materials (FSCMs) in extracted human molars. The single-section enamel specimens were prepared by slicing from the buccal to lingual surface including the FSCMs. After 5 weeks of automatic pH cycling, the demineralization was calculated by integrated mineral loss (ΔIML) from transverse-microradiography. The distributions of F and Sr were analyzed by the PIGE/PIXE technique. The micro-PIGE/PIXE technique indicated a fluorine uptake difference between the enamel surface and enamel cavity wall. AIML of FSCMs were significantly lower than intact enamel. The micro-PIGE/PIXE technique enables measurement of F and Sr uptake from FSCMs into enamel, which would be beneficial for research on caries development and prevention.
- Subjects
DENTAL enamel; TRACE elements; REMINERALIZATION (Teeth); X-ray emission spectroscopy; MICRORADIOGRAPHY; TRANSMISSION electron microscopy
- Publication
Dental Materials Journal, 2015, Vol 34, Issue 2, p240
- ISSN
0287-4547
- Publication type
Article
- DOI
10.4012/dmj.2014-127